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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
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Customer Service: (800) 227-6666 / (650) 697-3600
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Digoxigenin
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. Lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity (not to be confused with immunogenicity), that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Anti-digoxigenin antibodies with high affinities and specificity, are used in a variety of biological immuno-assays (e.g. ELISA). The antibodies are labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection. Digoxigenin is thus an all-purpose immuno-tag, and in particular a standard immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery.
Digoxigenin Target Details
| Target Name: | Digoxigenin |
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