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CRYBA1

crystallin, beta A1

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Gene Name: crystallin, beta A1
Synonyms: CRYBA1, Beta-crystallin A3, Crystallin, beta A1, CRYB1, Eye lens structural protein, Crystallin, beta A3
Target Sequences: NM_005208 NP_005199.2 P05813

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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
CRYBA1 (6)
No (6)
GST (1)
GST, N-terminus (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (4)
Mouse (1)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
CRYBA1 Protein - 12.5% SDS-PAGE of human CRYBA1 stained with Coomassie Blue
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Wheat Germ Extract
GST
51.5 kDa
10 µg/$479; 25 µg/$670
CRYBA1 Protein - Recombinant  Crystallin Beta A1 By SDS-PAGE
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E. coli
His-T7
28.9 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
CRYBA1 Protein - Purified recombinant protein CRYBA1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
25 kDa
20 µg/$1,107
CRYBA1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
CRYBA1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
25 kDa
20 µg/$150
CRYBA1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
25 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).