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BTD / Biotinidase

biotinidase

BTD / Biotinidase functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described.

Gene Name: biotinidase
Synonyms: BTD, Biotinase, Biotinidase
Target Sequences: NM_000060 NP_000051.1 P43251

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Products
Antibodies (30)
Primary (30)
BTD / Biotinidase (30)
Human (30)
Mouse (4)
Rat (4)
Monkey (1)
IHC (7)
IHC-P (4)
WB (23)
ELISA (7)
IP (1)
Peptide-ELISA (1)
rabbit (27)
mouse (2)
goat (1)
IgG (15)
IgG1,k (1)
monoclonal mc (2)
polyclonal pc (28)
3B10-2B3 (1)
APC (1)
APC Conjugated (2)
Biotin Conjugated (10)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (8)
aa42-271 (8)
aa372-385 (1)
No (30)
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BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, IHC, IHC-P, WB
Biotin Conjugated
100 µl/$412
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$341
BTD / Biotinidase Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
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BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
BTD / Biotinidase Antibody - Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
BTD / Biotinidase Antibody - Western Blot analysis of BTD expression in transfected 293T cell line by BTD monoclonal antibody (M01), clone 3B10-2B3.Lane 1: BTD transfected lysate(61.1 KDa).Lane 2: Non-transfected lysate.
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BTD / Biotinidase Mouse anti-Human Monoclonal (3B10-2B3) Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$423
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BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
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BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, IHC, IHC-P
Biotin Conjugated
100 µl/$412
BTD / Biotinidase Antibody - Immunohistochemical analysis of Biotinidase staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
IHC, IHC-P, WB
Biotin Conjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
BTD / Biotinidase Antibody - Western blot analysis of extracts of various cell lines, using BTD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
BTD / Biotinidase Antibody - Western blot analysis of extracts of various cell lines using BTD Polyclonal Antibody at dilution of 1:1000.
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BTD / Biotinidase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
BTD / Biotinidase Mouse anti-Human Monoclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
100 µl/$412
BTD / Biotinidase Antibody - BTD antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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BTD / Biotinidase Goat anti-Human Polyclonal (aa372-385) (Biotin) Antibody
Human, Monkey
Peptide-ELISA, WB
Biotin Conjugated
100 µg/$503
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) Antibody
Human
WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$338; 200 µl/$393; 500 µl/$575; 1 ml/$702; 2 ml/$939; 5 ml/$1,593
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$596; 2 ml/$824; 5 ml/$1,348
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) (APC) Antibody
Human
WB
APC Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) (PE) Antibody
Human
WB
PE Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
BTD / Biotinidase Antibody - Western blot of BTD / Biotinidase antibody.
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BTD / Biotinidase Rabbit anti-Human Polyclonal (aa42-271) (APC, Cy7) Antibody
Human
WB
APC, Cy7 Conjugated
100 µl/$505; 200 µl/$648; 500 µl/$1,152; 1 ml/$1,401; 2 ml/$1,961; 5 ml/$3,676
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
100 µl/$412
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µg/$749
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$937
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (APC) Antibody
Human
WB
APC Conjugated
100 µl/$937
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$937
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$937
Select
BTD / Biotinidase Rabbit anti-Human Polyclonal (PE) Antibody
Human
WB
PE Conjugated
100 µl/$937
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).