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BCKDHA / BCKDE1A

branched chain keto acid dehydrogenase E1, alpha polypeptide

The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
Synonyms: BCKDHA, BCKDH E1-alpha, BCKDE1A, MSU, MSUD1, OVD1A
Target Sequences: NM_000709 NP_000700.1 P12694

Publications (1)

1
SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis. Elma Zaganjor, Haejin Yoon, Jessica B Spinelli, Elizabeth R Nunn, Gaëlle Laurent, Paulina Keskinidis, Suganja Sivaloganathan, Shakchhi Joshi, Giulia Notarangelo, Stacy Mulei, Mathew T Chvasta, Sarah A Tucker, Krystle Kalafut, Robert A H van de Ven, Clary B Clish, Marcia C Haigis. Cell reports. 2021 July;36:109345. [Full Text Article] [PubMed:34260923] [PMC:PMC8320302]

☰ Filters
Antibodies (19)
Proteins (5)
ELISA Kits (2)
Over-Expression Lysate (2)
Primary (19)
Recombinant (3)
Sandwich (2)
BCKDHA / BCKDE1A (26)
Human (18)
Mouse (6)
Rat (6)
Bovine (3)
Chimpanzee (3)
Gibbon (3)
Guinea pig (3)
Horse (3)
Monkey (3)
Pig (3)
Rabbit (3)
Sheep (3)
Xenopus (3)
IHC (3)
IHC-P (5)
WB (15)
Flo (1)
ELISA (1)
ICC (2)
IF (3)
IP (2)
Peptide-ELISA (1)
rabbit (18)
mouse (1)
Product Group
IHCPlus (1)
PathPlus Other (1)
IgG (13)
polyclonal pc (18)
recombinant monoclonal rmc (1)
96-Well Strip Plate (2)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (13)
aa71-120 (4)
C-Terminus (1)
aa362-390 (1)
aa395-445 (1)
aa75-125 (1)
pSer292 (1)
No (26)
Plasma (2)
Serum (2)
Tissue Homogenates (2)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (2)
BCKDHA / BCKDE1A Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
BCKDHA / BCKDE1A Antibody - BCKDHA antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa71-120) Antibody
Human
WB
Unconjugated
100 µl/$424
BCKDHA / BCKDE1A Antibody - BCKDHA antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa71-120) (HRP) Antibody
Xenopus, Rabbit, Mouse, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Chimpanzee, Human, Monkey, Gibbon
WB
HRP Conjugated
100 µl/$469
BCKDHA / BCKDE1A Antibody - BCKDHA antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa71-120) (Biotin) Antibody
Xenopus, Rabbit, Mouse, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Chimpanzee, Human, Monkey, Gibbon
WB
Biotin Conjugated
100 µl/$469
BCKDHA / BCKDE1A Antibody - BCKDHA antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa71-120) (FITC) Antibody
Xenopus, Rabbit, Mouse, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Chimpanzee, Human, Monkey, Gibbon
WB
FITC Conjugated
100 µl/$469
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
BCKDHA / BCKDE1A Antibody
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µl/$286; 100 µl/$321
BCKDHA / BCKDE1A Antibody - Immunochemical staining of human BCKDHA in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, IHC-P, WB
Unconjugated
100 µl/$412
BCKDHA / BCKDE1A Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC-P, WB
Unconjugated
50 µl/$460
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BCKDHA / BCKDE1A Mouse anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µg/$705
BCKDHA / BCKDE1A Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCKDHA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa362-390) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
BCKDHA / BCKDE1A Antibody - Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa395-445) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
BCKDHA / BCKDE1A Antibody - Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from differentiated 3T3-L1 (3T3L1d) and 3T3-L1 (3T3L1) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho BCKDHA (S292) antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
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BCKDHA / BCKDE1A Rabbit anti-Mouse Polyclonal (pSer292) Antibody
Mouse
WB
Unconjugated
10 µl/$295; 100 µl/$533
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BCKDHA / BCKDE1A Rabbit anti-Human Polyclonal (aa75-125) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
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BCKDHA / BCKDE1A Rabbit Recombinant Monoclonal Antibody
WB
Unconjugated
20 µl/$299; 100 µl/$425
BCKDHA / BCKDE1A Protein
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E. coli
His
10 µg/$311; 50 µg/$498; 20 µg/$386; 100 µg/$790; 250 µg/$1,278; 500 µg/$1,917
BCKDHA / BCKDE1A ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
BCKDHA / BCKDE1A ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.312 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
BCKDHA / BCKDE1A Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
BCKDHA / BCKDE1A Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
50.4 kDa
20 µg/$150
BCKDHA / BCKDE1A Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
50.4 kDa
100 µg/$494
Viewing 1-25 of 26 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).