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ATP6V1D / V-ATPase subunit D

ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D

ATP6V1D / V-ATPase subunit D is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

Gene Name: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
Family/Subfamily: Transporter , ATPase - V type
Synonyms: ATP6V1D, ATP6M, V-ATPase subunit D, VPATPD, V-type proton ATPase subunit D, Vacuolar H-ATPase subunit D, Vacuolar proton pump D subunit, Vacuolar proton pump subunit D, VMA8, V-ATPase d subunit, Vacuolar ATPase subunit D, VATD
Target Sequences: NM_015994 NP_057078.1 Q9Y5K8

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Products
Antibodies (6)
Primary (6)
ATP6V1D / V-ATPase subunit D (6)
Human (6)
Mouse (3)
Rat (3)
IHC (2)
IHC-P (2)
WB (5)
Flo (1)
ELISA (3)
IF (1)
IP (1)
Peptide-ELISA (1)
rabbit (5)
mouse (1)
Product Group
IHCPlus (1)
IgG (5)
IgG2a,k (1)
monoclonal mc (1)
polyclonal pc (5)
3G4 (1)
Unconjugated (6)
70-150 aa, Internal (1)
No (6)
ATP6V1D / V-ATPase subunit D Antibody - Anti-ATP6V1D antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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ATP6V1D / V-ATPase subunit D Mouse anti-Human Monoclonal (3G4) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
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ATP6V1D / V-ATPase subunit D Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IHC, IP, WB
Unconjugated
100 µg/$331
ATP6V1D / V-ATPase subunit D Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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ATP6V1D / V-ATPase subunit D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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ATP6V1D / V-ATPase subunit D Rabbit anti-Human Polyclonal (70-150 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ATP6V1D / V-ATPase subunit D Antibody - Western blot analysis of extracts of various cell lines, using ATP6V1D antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
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ATP6V1D / V-ATPase subunit D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
ATP6V1D / V-ATPase subunit D Antibody - Immunochemical staining of human ATP6V1D in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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ATP6V1D / V-ATPase subunit D Rabbit anti-Human Polyclonal Antibody
Human
IHC-P
Unconjugated
100 µl/$412
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).