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ATP5B / ATP Synthase Beta

ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Family/Subfamily: Transporter , ATPase - F type
Synonyms: ATP5B, ATPMB, ATPSB, Beta-mtATPase, F0F1-ATP synthase beta subunit, ATP synthase beta subunit
Target Sequences: NM_001686 NP_001677.2 P06576

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Products
Proteins (10)
Over-Expression Lysate (2)
Recombinant (8)
ATP5B / ATP Synthase Beta (10)
No (10)
10His-SUMO, N-terminus + Myc, C-terminus (2)
6His, N-terminus (1)
GST (1)
His (1)
His-SUMO (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (5)
Mouse (3)
Rat (1)
293T Cells (1)
E. coli (4)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Yeast (2)
Purified (4)
ATP5B / ATP Synthase Beta Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
56.56 kDa
100 µg/$494
ATP5B / ATP Synthase Beta Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-SUMO, N-terminus + Myc, C-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
ATP5B / ATP Synthase Beta Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
10His-SUMO, N-terminus + Myc, C-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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293T Cells
Myc-DDK (Flag)
56.56 kDa
20 µg/$150
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HEK 293 Cells
Myc-DDK (Flag)
51.7 kDa
20 µg/$1,107
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E. coli
His-SUMO
67.7 kD
1 mg/$1,679; 100 µg/$500; 20 µg/$364
ATP5B / ATP Synthase Beta Protein - 12.5% SDS-PAGE of human ATP5B stained with Coomassie Blue
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Wheat Germ Extract
GST
83 kDa
10 µg/$479; 25 µg/$670
ATP5B / ATP Synthase Beta Protein - Recombinant  ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide By SDS-PAGE
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E. coli
His-T7
55.4 kDa
10 µg/$284; 50 µg/$460; 100 µg/$735; 200 µg/$917; 1 mg/$2,037; 500 µg/$1,571; 5 mg/$3,207; 2 mg/$2,225
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Yeast
His
53.7 kD
1 mg/$1,683; 100 µg/$499; 20 µg/$364
ATP5B / ATP Synthase Beta Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$446; 20 µg/$336; 1 mg/$1,460
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).