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ARID1B / BAF250B

AT rich interactive domain 1B (SWI1-like)

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Binds DNA non-specifically.

Gene Name: AT rich interactive domain 1B (SWI1-like)
Synonyms: ARID1B, 6A3-5, BRG1-associated factor 250b, BRG1-binding protein ELD/OSA1, DAN15, ELD/OSA1, ELD (eyelid)/OSA protein, KIAA1235, MRD12, HOsa2, OSA2, Osa homolog 2, RP11-419L10.1, BAF250B, BRG1-binding protein hELD/OSA1, p250R
Target Sequences: NM_020732 NP_065783.3 Q8NFD5

Publications (1)

1
Targeting ARID1A-mutant colorectal cancer: depletion of ARID1B increases radiosensitivity and modulates DNA damage response. Niedermaier B, Sak A, Zernickel E, Xu S, Groneberg M, Stuschke M. Science advances. 2019 December;9:18207. [Full Text Article] [PubMed:31796878] Related Antibodies: LS-C382223.

☰ Filters
Antibodies (15)
Proteins (3)
Over-Expression Lysate (2)
Primary (15)
Recombinant (1)
ARID1B / BAF250B (18)
Human (15)
Mouse (3)
Rat (6)
Pig (1)
IHC (8)
IHC-P (5)
WB (9)
ELISA (5)
ICC (2)
IF (9)
IP (2)
Peptide-ELISA (2)
rabbit (13)
mouse (2)
Product Group
IHCPlus (1)
IgG (12)
IgG2b,k (2)
monoclonal mc (2)
polyclonal pc (13)
2D2 (1)
2F2 (1)
Preservative Free (1)
Unconjugated (15)
aa1850-1900 (2)
1340-1420 aa, Internal (1)
Internal (1)
aa1300-1350 (1)
aa1371-1420 (1)
No (17)
Yes (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (2)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (1)
ARID1B / BAF250B Antibody - Anti-ARID1B / BRIGHT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ARID1B / BAF250B Mouse anti-Human Monoclonal (2D2) Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µg/$460
ARID1B / BAF250B Antibody - Western blot of BAF250b antibody
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (1340-1420 aa, Internal) Antibody
Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ARID1B / BAF250B Antibody - Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (aa1300-1350) Antibody
Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
ARID1B / BAF250B Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ARID1B / BAF250B Antibody - Immunoperoxidase of monoclonal antibody to ARID1B on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
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ARID1B / BAF250B Mouse anti-Human Monoclonal (2F2) Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
100 µg/$423
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ICC, IF, IHC-P
Unconjugated, Preservative Free
50 µl/$317; 100 µl/$375; 200 µl/$473
ARID1B / BAF250B Antibody - Peptide - + Immunofluorescence analysis of HUVEC cells, using BAF250B antibody.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Rat, Human
ELISA, IF, WB
Unconjugated
100 µg/$357
ARID1B / BAF250B Antibody - Immunofluorescence analysis of HUVEC cells, using BAF250B Antibody. The picture on the right is blocked with the synthesized peptide.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (aa1371-1420) Antibody
Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ARID1B / BAF250B Antibody - Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue  using ARID1B Polyclonal Antibody at dilution of 1:40(×200)
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ARID1B / BAF250B Antibody - Detection of Human ARID1B by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID1B used at a dilution of 1:1000 (1 ug/ml).
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (aa1850-1900) Antibody
Human
IHC, IP
Unconjugated
10 µg/$295; 100 µl/$514
ARID1B / BAF250B Antibody - Immunofluorescence analysis of C6 cells using ARID1B Polyclonal Antibody at dilution of 1:100.Blue: DAPI for nuclear staining.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
ARID1B / BAF250B Antibody - Detection of Human ARID1B by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B used at a dilution of 1:250.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal (aa1850-1900) Antibody
Human
IHC
Unconjugated
2.5 µg/$295; 100 µl/$532
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
20 µl/$275; 100 µl/$389
ARID1B / BAF250B Antibody - Immunochemical staining of human ARID1B in human esophagus with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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ARID1B / BAF250B Rabbit anti-Human Polyclonal Antibody
Human
IHC-P
Unconjugated
100 µl/$412
ARID1B / BAF250B Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
ARID1B / BAF250B Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
232.6 kDa
20 µg/$215
ARID1B / BAF250B Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
232.6 kDa
100 µg/$710
Viewing 1-18 of 18 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).