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ARHGAP35 / GRLF1

Rho GTPase activating protein 35

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.

Gene Name: Rho GTPase activating protein 35
Family/Subfamily: Ras GTPase superfamily IPR001806 , not assigned-Ras GTPase superfamily IPR001806
Synonyms: ARHGAP35, GRF-1, p190-A, p190A, p190ARhoGAP, Rho GAP p190A, p190RhoGAP, GRF1, GRLF1, KIAA1722
Target Sequences: NM_004491 NP_004482.4 Q9NRY4

☰ Filters
Antibodies (23)
Proteins (3)
Over-Expression Lysate (2)
Primary (23)
Recombinant (1)
ARHGAP35 / GRLF1 (26)
Human (23)
Mouse (20)
Rat (19)
Bat (3)
Bovine (3)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Guinea pig (3)
Horse (3)
Monkey (3)
Pig (4)
Rabbit (3)
IHC (8)
IHC-P (2)
WB (19)
ELISA (7)
IF (2)
IP (1)
Peptide-ELISA (6)
rabbit (22)
mouse (1)
IgG (13)
IgG2a,k (1)
monoclonal mc (1)
polyclonal pc (22)
4D4-F8 (1)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (20)
aa44-93 (4)
pTyr1105 (4)
pTyr1087 (3)
1040-1120 aa (2)
Ab-1105 (1)
Internal (1)
Tyr1105 (1)
aa1032-1081 (1)
aa1071-1120 (1)
aa1200-1250 (1)
No (26)
GST (1)
Myc-DDK (Flag) (2)
Human (2)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
ARHGAP35 / GRLF1 Antibody - Western blot of Phospho-GRF-1 (Y1105) antibody
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (1040-1120 aa) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ARHGAP35 / GRLF1 Antibody - 1:100 staining human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1105) Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ARHGAP35 / GRLF1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1105) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ARHGAP35 / GRLF1 Antibody - Western blot of GRF-1 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1105) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µg/$305; 100 µg/$357
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$688
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1105) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µg/$357
ARHGAP35 / GRLF1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (1040-1120 aa) Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ARHGAP35 / GRLF1 Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRF-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa1071-1120) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
ARHGAP35 / GRLF1 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using GRF-1 antibody.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (Ab-1105) Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
100 µg/$357
ARHGAP35 / GRLF1 Antibody - Western blot analysis of lysates from K562 cells, using phospho-GRF-1 (Phospho-Tyr1087) antibody.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1087) Antibody
Mouse, Rat, Human
ELISA, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ARHGAP35 / GRLF1 Antibody - Western blot of Phospho-GRF-1 (Y1087) antibody
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1087) Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ARHGAP35 / GRLF1 Antibody - Detection of Mouse GRLF1/p190RhoGAP by Western Blot. Samples: Whole cell lysate (50 ug) from CT26.WT, 4T1, and Renca cells. Antibodies: Affinity purified rabbit anti-GRLF1/p190RhoGAP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa1200-1250) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
ARHGAP35 / GRLF1 Antibody - Western blot analysis of lysates from MCF7 cells, using GRF-1 antibody.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa1032-1081) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
ARHGAP35 / GRLF1 Antibody - Detection limit for recombinant GST tagged GRLF1 is 0.1 ng/ml as a capture antibody.
Select
ARHGAP35 / GRLF1 Mouse anti-Human Monoclonal (4D4-F8) Antibody
Human
ELISA
Unconjugated
100 µg/$423
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µg/$305; 100 µg/$357
ARHGAP35 / GRLF1 Antibody - Western blot analysis of GRLF1 (pY1087) expression in HeLa (A); mouse brain (B) whole cell lysates.
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ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Dog, Rat, Pig, Human
WB
Unconjugated
100 µl/$315
ARHGAP35 / GRLF1 Antibody - Western blot of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml with Phospho-HUMAN-ARHGAP35 (Y1105) (upper) or Beta-actin (lower).
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (Tyr1105) Antibody
Human
WB
Unconjugated
400 µl/$393
ARHGAP35 / GRLF1 Antibody - GRLF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa44-93) Antibody
Human
WB
Unconjugated
100 µl/$424
ARHGAP35 / GRLF1 Antibody - GRLF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa44-93) (HRP) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
HRP Conjugated
100 µl/$469
ARHGAP35 / GRLF1 Antibody - GRLF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa44-93) (Biotin) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
ARHGAP35 / GRLF1 Antibody - GRLF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (aa44-93) (FITC) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Pig, Horse, Bat, Rat, Chimpanzee, Gibbon, Human, Monkey
WB
FITC Conjugated
100 µl/$469
ARHGAP35 / GRLF1 Antibody - Western blot of p-CD318 (Y707) pAb in extracts from K562 cells.
Select
ARHGAP35 / GRLF1 Rabbit anti-Human Polyclonal (pTyr1087) Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µl/$328; 100 µl/$419
Select
Wheat Germ Extract
GST
29.7 kDa
10 µg/$479; 25 µg/$670
ARHGAP35 / GRLF1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
172.18 kDa
20 µg/$215
Viewing 1-25 of 26 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).