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ACAT1

acetyl-CoA acetyltransferase 1

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Gene Name: acetyl-CoA acetyltransferase 1
Synonyms: ACAT1, ACAT, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase 1, MAT, T2, THIL
Target Sequences: NM_000019 NP_000010.1 P24752

Publications (2)

1
Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies. Chang CC, Chen J, Thomas MA, Cheng D, Del Priore VA, Newton RS, Pape ME, Chang TY. The Journal of biological chemistry. 1995 270:29532-40. [PubMed:7493995]
2
Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats. Song J, Kost CK, Martin DS. American journal of physiology. Regulatory, integrative and comparative physiology. 2006 290:R1608-15. [PubMed:16469840]

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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
ACAT1 (7)
No (7)
GST (2)
His (1)
His-S (1)
His-T7, N-terminus (1)
Myc-DDK (Flag) (2)
Human (5)
Mouse (1)
293T Cells (1)
E. coli (3)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (2)
ACAT1 Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
ACAT1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
45.6 kDa
20 µg/$215
ACAT1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
45.6 kDa
100 µg/$710
ACAT1 Protein - Recombinant Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) by SDS-PAGE
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E. coli
His-T7, N-terminus
37.2kDa
10 µg/$249; 50 µg/$307; 200 µg/$482; 100 µg/$385; 1 mg/$1,325; 500 µg/$962; 5 mg/$2,204; 2 mg/$1,480
ACAT1 Protein - Recombinant  Acetyl Coenzyme A Acetyltransferase 1 By SDS-PAGE
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E. coli
His-S
45.1 kDa
10 µg/$280; 50 µg/$437; 100 µg/$700; 200 µg/$872; 1 mg/$1,978; 500 µg/$1,519; 5 mg/$3,053; 2 mg/$2,164
ACAT1 Protein - 12.5% SDS-PAGE of human ACAT1 stained with Coomassie Blue
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Wheat Germ Extract
GST
41.91 kDa
10 µg/$479; 25 µg/$670
ACAT1 Protein - 12.5% SDS-PAGE of human ACAT1 stained with Coomassie Blue
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Wheat Germ Extract
GST
43.45 kDa
2 µg/$439
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).