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AANAT

aralkylamine N-acetyltransferase

AANAT belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: aralkylamine N-acetyltransferase
Synonyms: AANAT, AA-NAT, DSPS, Serotonin N-acetyltransferase, SNAT, Serotonin acetylase
Target Sequences: NM_001088 NP_001079.1 Q16613

Publications (5)

1
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation. Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC. The Journal of biological chemistry. 2001 276:24097-107. [PubMed:11313340]
2
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase. Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC. Investigative ophthalmology & visual science. 2002 43:564-72. [PubMed:11818405]
3
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin. Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F. Biochemical Society transactions. 2002 30:365-73. [PubMed:12196096]
4
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87). Coon SL, Del Olmo E, Young WS, Klein DC. The Journal of clinical endocrinology and metabolism. 2002 87:4699-706. [PubMed:12364461]
5
Arylalkylamine N-acetyltransferase: "the Timezyme". Klein DC. The Journal of biological chemistry. 2007 282:4233-7. [PubMed:17164235]
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Products
Antibodies (34)
Primary (34)
AANAT (34)
Human (24)
Mouse (5)
Rat (12)
Primate (2)
IHC (5)
IHC-P (1)
WB (27)
ELISA (10)
ICC (3)
IF (3)
rabbit (30)
mouse (4)
IgG (15)
IgG1 (4)
monoclonal mc (4)
polyclonal pc (30)
OTI3B9 (2)
OTI6E1 (2)
APC (1)
APC Conjugated (2)
Biotin Conjugated (4)
Carrier-free (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
DY488 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (3)
PE Conjugated (2)
Unconjugated (16)
aa22-235 (8)
aa27-54 (7)
aa1-72 (4)
aa22-236 (2)
Internal (1)
No (34)
AANAT Antibody - AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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AANAT Rabbit anti-Human Polyclonal Antibody
Human, Primate
IHC, WB
Unconjugated
0.1 ml/$386
AANAT Antibody - AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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AANAT Rabbit anti-Human Polyclonal (DY488) Antibody
Human, Primate
ICC, IHC, WB
DY488 Conjugated
0.1 ml/$416
AANAT Antibody - Immunohistochemistry (IHC) analysis of AANAT antibody in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) using PBS instead of primary antibody. Secondary antibody is Goat Anti-Rabbit IgG.
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AANAT Rabbit anti-Human Polyclonal Antibody
Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
AANAT Antibody - Immunohistochemistry of paraffin-embedded Human cervical cancer using AANAT Polyclonal Antibody at dilution of 1:40.
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AANAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
AANAT Antibody - Western blot analysis of AANAT expression in A549 (A); PC12 (B) whole cell lysates.
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AANAT Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
AANAT Antibody - Immunofluorescence staining of A549 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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AANAT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, WB
Unconjugated
50 µg/$294; 100 µg/$360
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AANAT Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
AANAT Antibody - Immunohistochemistry of paraffin-embedded Human brain using AANAT Polyclonal Antibody at dilution of 1:40.
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AANAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
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AANAT Rabbit anti-Mouse Polyclonal (aa22-236) (Biotin) Antibody
Mouse
ICC, WB
Biotin Conjugated
100 µl/$316
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AANAT Rabbit anti-Mouse Polyclonal (aa22-236) (FITC) Antibody
Mouse
ICC, WB
FITC Conjugated
100 µl/$325
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AANAT Rabbit anti-Human Polyclonal (aa27-54) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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AANAT Rabbit anti-Human Polyclonal (aa27-54) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
200 µl/$952
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AANAT Rabbit anti-Human Polyclonal (aa27-54) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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AANAT Rabbit anti-Human Polyclonal (aa27-54) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
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AANAT Rabbit anti-Human Polyclonal (aa27-54) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
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AANAT Rabbit anti-Human Polyclonal (aa27-54) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
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AANAT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$331
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AANAT Rabbit anti-Human Polyclonal Antibody
Human
IF
Unconjugated
20 µl/$275; 100 µl/$389
AANAT Antibody - Immunofluorescence analysis of U2OS cells using AANAT Polyclonal Antibody at dilution of 1:100.Blue: DAPI for nuclear staining.
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AANAT Rabbit anti-Human Polyclonal Antibody
Human
IF
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
AANAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed
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AANAT Mouse anti-Human Monoclonal (aa1-72) (OTI6E1) Antibody
Human
WB
Unconjugated
100 µl/$434
AANAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT. (1:2000)
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AANAT Mouse anti-Human Monoclonal (aa1-72) (Carrier-free) (OTI6E1) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
AANAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed
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AANAT Mouse anti-Human Monoclonal (aa1-72) (OTI3B9) Antibody
Human
WB
Unconjugated
100 µl/$434
AANAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY AANAT. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT. (1:2000)
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AANAT Mouse anti-Human Monoclonal (aa1-72) (Carrier-free) (OTI3B9) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
AANAT Antibody - Western blot of AANAT Antibody in Y79 cell line lysates (35 ug/lane). AANAT (arrow) was detected using the purified antibody.
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AANAT Rabbit anti-Human Polyclonal (aa27-54) Antibody
Human
WB
Unconjugated
400 µl/$393
AANAT Antibody - Western blot of AANAT antibody.
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AANAT Rabbit anti-Rat Polyclonal (aa22-235) Antibody
Rat
WB
Unconjugated
100 µl/$311; 200 µl/$355; 500 µl/$499; 1 ml/$571; 2 ml/$777; 5 ml/$1,261
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).