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| Catalog Number | Size | Price |
|---|---|---|
| LS-G51300-20 | 20 µg | $294 |
| LS-G51300-100 | 100 µg (0.25 mg/ml) | $426 |
| LS-G51300-1 | 1 mg | $1,869 |
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Mouse IL6ST / CD130 / gp130 Protein (Recombinant His,C-terminus) (Met1-Glu617) - LS-G51300
Mouse IL6ST / CD130 / gp130 Protein (Recombinant His,C-terminus) (Met1-Glu617) - LS-G51300
Description:
IL6ST / CD130 / gp130 Protein LS-G51300 is a Recombinant Mouse IL6ST / CD130 / gp130 produced in HEK 293 Cells Met1-Glu617 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IL6ST / CD130 / gp130 Protein LS-G51300 is a Recombinant Mouse IL6ST / CD130 / gp130 produced in HEK 293 Cells Met1-Glu617 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL6ST / CD130 / gp130
Synonyms
IL6ST | CD130 | CD130 antigen | IL-6R subunit beta | IL-6 receptor subunit beta | Membrane glycoprotein gp130 | Membrane glycoprotein 130 | IL-6RB | CDW130 | gp130 | IL-6R-beta
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Glu617
Predicted Molecular Weight
The secreted recombinant mouse gp130 consists of 606 amino acids and has a predicted molecular mass of 68 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 97 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to bind mouse IL11Ra in a functional ELISA. Measured by its ability to inhibit the IL-6Ra enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 µg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL6ST / CD130 / gp130
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Functional Assay
Measured by its ability to inhibit the IL-6Ra enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 µg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to inhibit the IL-6Ra enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 µg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to inhibit the IL-6Ra enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 µg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Functional Assay
Measured by its ability to inhibit the IL-6Ra enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 µg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular IL6ST / CD130 / gp130 Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
6His, N-terminus
Source:
E. coli
Tag:
His
Source:
Human
Tag:
His, N-Terminal
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/19/2024
Date Requested: 11/19/2024
