Target
LDLR / LDL Receptor
Synonyms
LDLR | FHC | LDL receptor | LDLCQ2
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The secreted recombinant mouse LDLR comprises 780 amino acids with a predicted molecular mass of 85.7 kDa. As a result of glycosylation, it migarates as approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10 µg/ml (100 µl/well) can bind biotinylated recombinant mouse LDLR. The EC50 of biotinylated mouse LDLR is 0.173 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About LDLR / LDL Receptor