Target
CCNE1 / Cyclin E1
Synonyms
CCNE1 | Cyclin E1 | CCNE | Cyclin Es | Cyclin Et | G1/S-specific cyclin-E1 | G1/s-specific cyclin e | Cyclin E
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
Expression System
Baculovirus
Purification
> 82 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 µg/ml (100 µl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, 1mM GSH, pH 7.4, 5% trehalose, 5% mannitol 0.01% Tween80.
Reconstitution
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee