Target
WNT3A
Synonyms
WNT3A | Protein Wnt-3a
Conjugations
Unconjugated
Predicted Molecular Weight
38.0-41.0 kDa
AA Sequence
SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK
Expression System
Cell Culture
Purification
Greater than 75% by SDS-PAGE and HPLC
Bio-Activity
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 [Character 200b]for this effect is 0.4-0.6 ng/ml.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
10mM Sodium Phosphate pH 7.5, 20mM Potassium Chloride , 500 mM NaCl + 0.5% CHAPS, pH 7.5.
Reconstitution
Reconstitute in water to <0.1 mg/ml. Do not vortex.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee