Target
ITGAX + ITGB2 Heterodimer
Conjugations
Unconjugated
Predicted Molecular Weight
205 kDa
Expression System
HEK 293 Cells
Purification
Greater than 90% by SDS-PAGE
Usage Summary
A DNA sequence encoding the extracellular domain (Met 1-Pro 1107) of human ITGAX (P20702) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asn 700) of human ITGB2 (P05107) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration up to 0.25mg/mL. Do not vortex.
Storage
Store lyophilized at -20°C to -80°C for up to 1 year. Aliquot and store reconstituted at 4°C to 8°C for up to 1 week, or at -20°C for up to 3 months. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee