Target
CCN4 / WISP1
Synonyms
WISP1 | CCN4 | WISP1i | WISP1tc | Wnt-1-induced secreted protein | WISP-1 | WISP1c | CCN family member 4
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 345 amino acids recombinant protein has a predicted molecular mass of 38.1 kD. The predicted N-terminal amino acid is Thr.
Purification
Greater than 98% by SDS-PAGE and HPLC
Usage Summary
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Slow to dissolve. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0 - 3.0 µg/ml.
Endotoxin
Less than 0.1 ng/µg of protein.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Additional carrier protein (example 0.1% BSA) is recommended for long term storage.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee