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| Catalog Number | Size | Price |
|---|---|---|
| LS-G137547-10 | 10 µg (0.2 mg/ml) | $344 |
| LS-G137547-25 | 25 µg (0.2 mg/ml) | $459 |
| LS-G137547-100 | 100 µg | $1,000 |
| LS-G137547-500 | 500 µg | $2,235 |
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Mouse IL-33 Protein (Recombinant) (Ser109-Ile266) - LS-G137547
Mouse IL-33 Protein (Recombinant) (Ser109-Ile266) - LS-G137547
Description:
IL-33 Protein LS-G137547 is a Recombinant Mouse IL-33 produced in E. coli Ser109-Ile266. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IL-33 Protein LS-G137547 is a Recombinant Mouse IL-33 produced in E. coli Ser109-Ile266. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Species
Mouse
Modifications
Carrier-free
Conjugations
Unconjugated
Region
Ser109-Ile266
Predicted Molecular Weight
The 158 amino acid recombinant protein has a predicted molecular mass of approximately 17,554 Da. The DTT-reduced and non-reduced protein migrate at approximately 20 kDa by SDS-PAGE. The N-terminal amino acid is Serine.
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50 - 100 µg/ml in sterile buffer (HBS) containing carrier protein such as 0.2 - 1% BSA or HSA.
Bio-Activity
ED50 is equal to0.0125 - 0.0625 ng/ml determined by the dose dependent stimulation of D10.G4.1 cell proliferation.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 20 mM HEPES, pH 7.2, 150 mM NaCl, 10 mM TCEP
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL-33
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Images
Proliferation
Mouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner.
Functional Assay
IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Proliferation
Mouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner.
Functional Assay
IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Proliferation
Mouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner.
Functional Assay
IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Proliferation
Mouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner.
Functional Assay
IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Popular IL-33 Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
