Target
LGALS3 / Galectin 3
Synonyms
LGALS3 | 35 kd lectin | 35 kDa lectin | CBP35 | GAL3 | Galactoside-binding protein | GALIG | IgE-binding protein | Laminin-binding protein | Gal-3 | GALBP | MAC-2 | Mac-2 antigen | L31 | MAC2 | CBP 35 | Galactose-specific lectin 3 | Galectin-3 | L-31 | Lectin L-29
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
This 284 amino acid recombinant protein has a predicted molecular mass of approximately 29.5 kD. The protein migrates as above 45 kD in SDS-PAGE in DTT-reducing and non-reducing conditions. The predicted N-terminal amino acid is His.
Expression System
HEK 293 Cells
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA.
Bio-Activity
Recombinant mouse galectin-3 is able to agglutinate human red blood cells with an ED50 of 2.0 - 8.0 µg/ml, corresponding to a specific activity of 2.5 - 10.0 x 10^5 units/mg.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 10 mM NaH2PO4, 1 mM EDTA, 0.3 M NaCl, 2 mM DTT
Storage
Store unopened at 2°C to 8°C for up to 3 months, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at 2°C to 8°C for up to 1 month, or at -20°to -70°C for up to 3 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About LGALS3 / Galectin 3