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| Catalog Number | Size | Price |
|---|---|---|
| LS-G49851-50 | 50 µg | $403 |
| LS-G49851-500 | 500 µg | $1,697 |
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Mouse IL23 Protein (Recombinant His,C-terminus) (Met1-Ala196) - LS-G49851
Mouse IL23 Protein (Recombinant His,C-terminus) (Met1-Ala196) - LS-G49851
Description:
IL23 Protein LS-G49851 is a Recombinant Mouse IL23 produced in HEK 293 Cells Met1-Ala196 + Met1-Ser335 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
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(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IL23 Protein LS-G49851 is a Recombinant Mouse IL23 produced in HEK 293 Cells Met1-Ala196 + Met1-Ser335 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL23
Synonyms
IL23
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Ala196 + Met1-Ser335
Predicted Molecular Weight
The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
(33.1+31.5+32.9) % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 µg/ml. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 µg/ml. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 21.3-49.8 ng/mL. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Binding
Measured by its binding ability in a functional ELISA. 1. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 µg/ml. 3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 µg/ml. 4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 21.3-49.8 ng/mL. 5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. 1. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 µg/ml. 3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 µg/ml. 4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 21.3-49.8 ng/mL. 5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. 1. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 µg/ml. 3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 µg/ml. 4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 21.3-49.8 ng/mL. 5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. 1. Measured by its ability to bind recombinant rat IL23R-Fc in a functional ELISA. 2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 µg/ml. 3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.05-0.11 µg/ml. 4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 21.3-49.8 ng/mL. 5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular IL23 Proteins
Source:
Human
Tag:
His,C-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/14/2025
Date Requested: 1/14/2025
