Recombinant Protein

IL12

Mouse

Unmodified

75.0 kDa

CHO Cells

Hamster-Chinese

Greater than 98% by SDS-PAGE and HPLC

Assay 1: The ED50 as determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7 units/mg. Assay 2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is =0.1ng/ml, corresponding to a specific activity of =1 x 10^7 units/mg.

Less than 0.1 ng/µg of protein (less than 1EU/µg).

Sterile filtered, lyophilized from 1X PBS, pH 7.2

Centrifuge the vial prior to opening. Reconstitute in 1X PBS pH 7.2-7.4 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.