Target
IFN Beta / Interferon Beta
Synonyms
IFNB1 | IFF | IFB | IFNB | Interferon beta | Interferon, beta 1, fibroblast | Fibroblast interferon | IFN-beta
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 175 amino acid recombinant protein has a predicted molecular mass of approximately 21.2 kD. The DTT-reduced and non-reduced protein migrates at approximately 36 kD by SDS-PAGE. The N-terminal amino acid is Ile.
Expression System
HEK 293 Cells
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Mouse IFN-ß1 induces the production of mouse CCL2 in RAW 264.7 mouse monocyte/macrophage cells in a dose-dependent manner. The ED50 for this effect is 0.9 – 4.5 ng/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About IFN Beta / Interferon Beta