Target
HMG1 / HMGB1
Synonyms
HMGB1 | Amphoterin | High mobility group protein B1 | High mobility group box 1 | High mobility group protein 1 | HMG1 | HMG3 | SBP-1 | High-mobility group box 1 | HMG-1
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG1 Fc, N-terminus
Predicted Molecular Weight
The recombinant mouse HMGB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 475 amino acids and has a calculated molecular mass of 53.3 kDa. The apparent molecular mass of the monomer is approximately 57 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 85 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to bind mouse AGER-His in functional ELISA.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80
Reconstitution
It is recommended that sterile water (330ul) be added to the vial to prepare a stock solution of 0.15mg/mL.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee