Target
GZMB / Granzyme B
Synonyms
GZMB | CCPI | CGL-1 | Cathepsin G-like 1 | CSP-B | CTLA-1 | CTLA1 | CGL1 | CSPB | Fragmentin 2 | GRB | HLP | Human lymphocyte protein | Fragmentin-2 | SECT | Lymphocyte protease | T-cell serine protease 1-3E | C11 | CTSGL1 | Cytotoxic serine protease B | Granzyme B | Granzyme-2
Conjugations
Unconjugated
Predicted Molecular Weight
28.9 kDa
AA Sequence
IIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS
Expression System
Baculovirus
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per ug of enzyme.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 1X PBS
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee