Recombinant Protein

FGF1 / Acidic FGF

Mouse

Unmodified

15.9 kDa

E. coli

E. coli

Greater than 95% by SDS-PAGE and HPLC

Assay 1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is = 0.5 ng/ml corresponding to a specific activity of = 2 x 10^6 units/mg. Assay 2: The ED50 as determined by a cell proliferation Assay using balb/c 3T3 cells is = 0.2 ng/ml in the presence of 10 µg/ml heparin, corresponding to a specific activity of = 5 x 10^6 units/mg.

Less than 0.1 ng/µg of protein (less than 1EU/µg).

Sterile filtered, lyophilized from 5 mM sodium phosphate, pH 7.2, 100 mM sodium chloride

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate pH 7.0-7.2 to a concentration of 0.5-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P05230 NCBI: X59065 P05230