Target
DLL1
Synonyms
DLL1 | Delta-like 1 (Drosophila) | Delta (Drosophila)-like 1 | Drosophila Delta homolog 1 | Delta-like protein 1 | Delta1 | H-Delta-1 | DL1 | Npa
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant mouse DLL1 consists of 506 amino acids and has a predicted molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm DLL1 is approximately 65 kDa due to glycosylation.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its ability to bind human NOTCH1 in a functional ELISA. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, 20% glycerol, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee