Target
CXCL1 / GRO Alpha
Synonyms
CXCL1 | C-X-C motif chemokine 1 | Fibroblast secretory protein | FSP | GRO1 | GROa | Growth-regulated alpha protein | GRO-alpha(1-73) | MGSA | MGSA-a | Small inducible cytokine b1 | GRO | MGSA alpha | NAP-3 | SCYB1
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.4 kD. The DTT-reduced protein migrates at approximately 10 kD and non-reduced protein migrates at slightly higher than 10 kD by SDS-PAGE. The N-terminal amino acid is
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant mouse CXCL1 (KC) chemoattracts Baf3-mCXCR2 transfectants in a dose-dependent manner. The ED50 for this effect is 0.1– 0.6 ng/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee