Recombinant Protein

c-Met

Mouse

Unmodified

The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved a and ß subunits. Each a and ß together with the C-terminal Fc tag consists of 1146 amino acids and has a predicted molecular mass of 128 (a =32 + Fc tagged ß=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.

HEK 293 Cells

Human

> 92 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 µg/ml (100 µl/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to included instructions for detailed information.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P08581 NCBI: NM_000245 NP_000236.2