Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Region
Met1-Pro189 + Met1-Asn328
Predicted Molecular Weight
The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE.
Expression System
HEK 293 Cells
Purification
> 85 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized marmoset IL23A-His+marmoset IL12B-His at 10 µg/ml (100 µl/well) can bind biotinylated human IL12RB1-His. The EC50 of biotinylated human IL12RB1-His is 55-129 µg/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee