Target
WNT1
Synonyms
WNT1 | INT1 | Proto-oncogene Int-1 homolog | Proto-oncogene Wnt-1
Conjugations
Unconjugated
Predicted Molecular Weight
~38.5 kDa
Expression System
E. coli
Source Species
Escherichia coli
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200ng/ml of human BMP-2.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile Tris, DTT
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
The lyophilized protein is stable for up to 1 year at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°C to 8°C for 1 month, or at -20°C for 6 months, with a carrier protein without detectable loss of activity. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee