Target
TNFRSF1A / TNFR1
Synonyms
TNFRSF1A | CD120a | CD120a antigen | MS5 | p60 | TBP1 | TNF-R55 | TNFAR | TNFR1 | p55-R | TNF-R | TNF-R-I | TNF-R1 | TNF-RI | Tnfr | TNFR-I | TNFR1-d2 | TNFR60 | FPF | p55 | Tbpi | TNFR55
Conjugations
Unconjugated
Predicted Molecular Weight
18.3 kDa
AA Sequence
MDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIEN
Expression System
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha, is 0.05 ug/ml.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 8.0, 50 mM NaCl
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee