Target
S100B / S100 Beta
Synonyms
S100B | NEF | Protein S100-B | S100 | S100 calcium binding protein B | S100 calcium-binding protein B | S100beta | S-100 protein beta chain | S-100 protein subunit beta | S100-B
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG1 Fc, N-terminus
Predicted Molecular Weight
The recombinant human Fc/S100B is a disulfide-linked homodimeric protein. The reduced monomer consists of 328 amino acids and has a predicted molecular mass of 37.2 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100B monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its ability to bind mouse S100A1 in a functional ELISA. Measured by its ability to bind TP53 in a functional ELISA. Immobilized recombinant human Fc-S100B at 10 µg/mL (100 µl/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee