Target
PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Conjugations
Biotin.
Also available
Unconjugated.
Region
Extracellular domain of human CD279(PD-1) and murine IgG2a Fc +hinge.
Predicted Molecular Weight
52 kDa
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble fusion protein consisting of the extracellular (139aa) domain of human CD279(PD-1) and murine IgG2a Fc +hinge. In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions. Data suggest that the murine Ig Fc portion of this construct may be missing. Five x 10^5cultured human monocyte-derived Dendritic cells (moDC) were washed and incubated 45 minutes on ice with 80 ul of CD279-muIg/Biotin at 10 µg/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.57 log10 fluorescent units. This binding was blocked when reagent was pre incubated with 100 ug/ml of purified CD273-muIg.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 2°C to 5°C for up to 6 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1