Target
NTF3 / Neurotrophin 3
Synonyms
NTF3 | HDNF | NGF2 | Nerve growth factor 2 | NGF-2 | NT-3 | Neurotrophic factor | Neurotrophin 3 | Neurotrophin-3
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
NT-3 recombinant protein is a noncovalent linked homodimer. The 120 amino acid recombinant protein has a predicted molecular mass of approximately 13.6 kD as monomer, 26.5 kD as dimer. The predicted N-terminal amino acid is Met.
Purification
Greater than 98% by SDS-PAGE and HPLC
Usage Summary
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA.
Bio-Activity
The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 10^4 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein.
Storage
Store unopened at -20°C to -70°C. Store diluted at -20°C to -80°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About NTF3 / Neurotrophin 3