Target
MME / CD10
Synonyms
MME | Atriopeptidase | CALLA | CD10 | EPN | Membrane metallo-endopeptidase | NEP | Neprilysin | Neutral endopeptidase | Neutral endopeptidase 24.11 | CD10 antigen | Enkephalinase | Neprylisin | SFE | Skin fibroblast elastase
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human MME consists of 699 amino acids and predicts a molecular mass of 80 kDa. As a result of glycosylation, rhMME migrates as approximately 90-100 kDa band in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp)-OH. The specific activity is >1,000 pmoles/min/µg.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee