Target
MFGE8 /Lactadherin
Synonyms
MFGE8 | Breast epithelial antigen BA46 | EDIL1 | HP47 | Lactadherin | Medin | MFGM | Milk fat globule-EGF factor 8 | HsT19888 | OAcGD3S | SPAG10 | Sperm associated antigen 10 | MFG-E8 | SED1 | Sperm surface protein hP47 | BA46 | HMFG
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The secreted recombinant human MFGE8 comprises 374 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh MFGE8 is approximately 45 kDa in SDS-PAGE under reducing conditions.
Expression System
Baculovirus
Purification
> 80 % as determined by SDS-PAGE
Bio-Activity
When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 µg/mL, 100 µL/well), 45-85% cells will adhere after 1 hour at 37°C.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee