Target
Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Conjugations
Unconjugated
Predicted Molecular Weight
16.0 kDa
AA Sequence
MVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Expression System
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
This Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 ug Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 0.1% TFA.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0-5.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee