Target
Leptin
Synonyms
LEP | Leptin | LEPD | Obese protein | Obese, mouse, homolog of | Obesity factor | OBS | OB
Conjugations
Unconjugated
Predicted Molecular Weight
16.0 kDa
AA Sequence
VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
Purification
Greater than 97% by SDS-PAGE and HPLC
Usage Summary
A single non-glycosylated polypeptide chain containing 146 amino acids.
Bio-Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4
Reconstitution
Briefly centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage
Store lyophilized at 2°C to 8°C for short term storage. Store lyophilized at -20°C for long term storage. Store reconstituted at 2°C to 8°C for up to 1 week. Aliquot and store reconstituted at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee