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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47860-100 | 100 µg | $519 |
| LS-G47860-1 | 1 mg | $2,551 |
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Human IL2RB / CD122 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Asp239) - LS-G47860
Human IL2RB / CD122 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Asp239) - LS-G47860
Description:
IL2RB / CD122 Protein LS-G47860 is a Recombinant Human IL2RB / CD122 produced in HEK 293 Cells Met1-Asp239 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IL2RB / CD122 Protein LS-G47860 is a Recombinant Human IL2RB / CD122 produced in HEK 293 Cells Met1-Asp239 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL2RB / CD122
Synonyms
IL2RB | CD122 | CD122 antigen | IL-2 receptor subunit beta | IL15RB | Interleukin 15 receptor, beta | p70-75 | IL-2R subunit beta | IL-2RB | Interleukin 2 receptor, beta
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG Fc, C-terminus
Region
Met1-Asp239
Predicted Molecular Weight
The recombinant human IL2 Rß/Fc is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51.6 kDa. As a result of glycosylation, the apparent molecular mass of rh IL2RB/Fc monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Using the Octet RED System, the affinity constant (Kd) of Human IL2RB-Fc bound to Human IL2 was 0.8nM.2. Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL2RB / CD122
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Binding
Using the Octet RED System, the affinity constant (Kd) of CD122 / IL2RB Protein, Human, Recombinant (Fc Tag) bound to IgG1 Antibody was 0.3 µM.
Proliferation
Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 µg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD122 / IL2RB Protein, Human, Recombinant (Fc Tag) bound to IgG1 Antibody was 0.3 µM.
Proliferation
Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 µg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD122 / IL2RB Protein, Human, Recombinant (Fc Tag) bound to IgG1 Antibody was 0.3 µM.
Proliferation
Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 µg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD122 / IL2RB Protein, Human, Recombinant (Fc Tag) bound to IgG1 Antibody was 0.3 µM.
Proliferation
Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 µg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular IL2RB / CD122 Proteins
Source:
E. coli
Tag:
6His, N-terminus
Source:
E. coli
Tag:
His, N-Terminal
Source:
Human
Tag:
6His, C-terminus
Source:
Human
Tag:
Fc, C-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
