Recombinant Protein

IL2RB / CD122

Human

Unmodified

The mature recombinant human IL2 Rß consists of 219 amino acids and has a predicted molecular mass of 25.2 kDa. As a result of glycosylation, the rh IL2 Rß migrates as an approximately 40 kDa protein in SDS-PAGE under reducing conditions.

HEK 293 Cells

Human

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 µg/ml (100 µl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 µg/mL. Measured by its ability to inhibit IL15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1-4µg/mL.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to included instructions for detailed information.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P14784 NCBI: NM_000878 NP_000869.1