Target
IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Conjugations
Unconjugated
Tag
Human IgG1 Fc, C-terminus
Predicted Molecular Weight
65-75 kDa
AA Sequence
ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSCexpressedwithaC-terminalhumanIgG1Fcfragment
Expression System
HEK 293 Cells
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Immobilized Human IL-2 at 5µg/mL (100 µL/well) can bind Human CD25/IL-2Ra, Fc Chimera with a linear range of 1.2-11 ng/mL.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered solution in PBS
Reconstitution
Reconstitute in ddH2O or PBS at 100 ug/ml.
Storage
Store lyophilized at -70°C or below for up to 6 months. Store reconstituted at 4°C for up to 1 week or at -20°C for up to 3 months. Add a carrier protein (0.1% BSA) for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee