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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47278-100 | 100 µg | $403 |
| LS-G47278-1 | 1 mg | $1,697 |
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Human IL2RA / CD25 Protein (Recombinant His,C-terminus) (Met1-Cys213) - LS-G47278
Human IL2RA / CD25 Protein (Recombinant His,C-terminus) (Met1-Cys213) - LS-G47278
Description:
IL2RA / CD25 Protein LS-G47278 is a Recombinant Human IL2RA / CD25 produced in HEK 293 Cells Met1-Cys213 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IL2RA / CD25 Protein LS-G47278 is a Recombinant Human IL2RA / CD25 produced in HEK 293 Cells Met1-Cys213 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Cys213
Predicted Molecular Weight
The recombinant human IL2Ra consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Ra is approximately 44 kDa due to glycosylation.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 97 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to inhibit IL2-induced proliferation of CTLL2 cells. The ED50 for this effect is 5-20 µg/mL in the presence of 1 ng/mL of recombinant human IL-2.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL2RA / CD25
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Proliferation
Measured by its ability to inhibit IL2-induced proliferation of CTLL2 cells. The ED50 for this effect is 5-20 µg/mL in the presence of 1 ng/mL of recombinant human IL-2.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured by its ability to inhibit IL2-induced proliferation of CTLL2 cells. The ED50 for this effect is 5-20 µg/mL in the presence of 1 ng/mL of recombinant human IL-2.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured by its ability to inhibit IL2-induced proliferation of CTLL2 cells. The ED50 for this effect is 5-20 µg/mL in the presence of 1 ng/mL of recombinant human IL-2.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured by its ability to inhibit IL2-induced proliferation of CTLL2 cells. The ED50 for this effect is 5-20 µg/mL in the presence of 1 ng/mL of recombinant human IL-2.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular IL2RA / CD25 Proteins
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Source:
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Source:
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Tag:
His, N-Terminal
Source:
Human
Tag:
6His, C-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
