Target
IL27
Synonyms
IL27 | IL-27 | IL-27 p28 subunit | IL30 | Interleukin 27 | Interleukin-27 subunit alpha | IL27A | Interleukin 30 | IL-27-A | IL27p28 | IL-27 subunit alpha | IL-27A | IL27-A | p28
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The total predicted molecular weight is 50 kD. The non-reduced and the DTT-reduced proteins migrate as one band at approximately 60 kD by SDS-PAGE.
Expression System
CHO Cells
Source Species
Hamster-Chinese
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant Human IL-27 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 1.5 - 9 ng/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution is in 20 mM HEPES, 300 mM NaCl, 2 mM CHAPS, pH 7.2
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee