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| Catalog Number | Size | Price |
|---|---|---|
| LS-G257-20 | 20 µg | $268 |
| LS-G257-100 | 100 µg | $349 |
Human IFN Gamma / Interferon Gamma Protein (Recombinant) - LS-G257
Human IFN Gamma / Interferon Gamma Protein (Recombinant) - LS-G257
Description:
IFN Gamma / Interferon Gamma Protein LS-G257 is a Recombinant Human IFN Gamma / Interferon Gamma produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
IFN Gamma / Interferon Gamma Protein LS-G257 is a Recombinant Human IFN Gamma / Interferon Gamma produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IFN Gamma / Interferon Gamma
Synonyms
IFNG | IFG | Immune interferon | IFI | Interferon gamma | IFN-gamma | Interferon, gamma | IFNY
Species
Human
Modifications
Unmodified.
Also available Animal-free.
Conjugations
Unconjugated
Predicted Molecular Weight
16.8 kDa
AA Sequence
MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
Assay 1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay 2: The ED50 as determined by a cytotoxicity Assay using HT-29 cells is = 0.05 ng/ml, corresponding to a specific activity of = 2 x 10^7 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IFN Gamma / Interferon Gamma
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
