Target
ICOS / CD278
Synonyms
ICOS | AILIM | CD278 | CVID1 | Inducible costimulator | Inducible T-cell co-stimulator | CD278 antigen | Inducible T-cell costimulator
Conjugations
Phycoerythrin.
Also available
Unconjugated or conjugated with
Biotin.
Region
Extracellular domain of mature human ICOS fused to the murine IgG2a Fc.
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Five x 10^5 cultured human Raji cells cells per tube were washed and incubated 45 minutes on ice with 80 µl of CD278(ICOS)-muIg/PE at a 1:20 dilution (10µg/ml). They were then washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control. Binding was blocked when reagent was pre incubated 10 minutes with a 5-fold excess of CD275(ICOSL)-muIg.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 2°C to 5°C for up to 1 year. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee