Recombinant Protein

ICOS / CD278

Human

Preservative Free. Also available Unmodified.

42.8 kDa

CHO Cells

Hamster-Chinese

A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8 kd (amino acid composition only). ICOS-muIg fusion protein binds to Raji cells in FACS. Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 µl of ICOS-muIg at 5 µg/ml. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.42 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Binding was blocked when reagent was pre incubated with 100 µg/ml of recombinant GL50(ICOSL)-muIg. ICOS-muIg fusion protein was reactive in EIA using a Goat-anti-mouse-Ig antibody capture, followed by detection using GL50-muIg/Biotin and Streptavidin/HRP. Amino acid sequence of fusion protein was confirmed by n-terminal analysis (EINGS).

Not Tested

Not Tested

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl

Store at 2°C to 5°C for up to 1 year. Avoid freeze-thaw cycles. Open under aseptic conditions.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: Q9Y6W8 NCBI: AJ277832 CAC06612.1