Target
HMG1 / HMGB1
Synonyms
HMGB1 | Amphoterin | High mobility group protein B1 | High mobility group box 1 | High mobility group protein 1 | HMG1 | HMG3 | SBP-1 | High-mobility group box 1 | HMG-1
Conjugations
Unconjugated
Applications
- Binding
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human HMGB1 consists of 117 amino acids and predicts a molecular mass of 13.6 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions.
Expression System
E. coli
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Immobilized human His-HMGB1 at 10 µg/ml (100 µl/well) can bind AGER-Fc with a linear range of 0.31-2.5 µg/ml.
Presentation
Lyophilized from sterile PBS, 20% Glycerol, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee