Target
FGF20
Synonyms
FGF20 | Fibroblast growth factor 20 | FGF-20
Conjugations
Unconjugated
Predicted Molecular Weight
23.2 kDa
AA Sequence
PLAEVGGFLGGLEGLGQQVGSHFLLPPAGERPPLLGERRSAAERSARGGPGAAQLAHLHGILRRRQLYCRTGFHLQILPDGSVQGTRQDHSLFGILEFISVAVGLVSIRGVDSGLYLGMNDKGELYGSEKLTSECIFREQFEENWYNTYSSNIYKHGDTGRRYFVALNKDGTPRDGARSKRHQKFTHFLPRPVDPERVPELYKDLLMYT
Expression System
E. coli
Purification
Greater than 90% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by a Balb/c 3T3 cell proliferation assay is = 10 ng/ml, corresponding to a specific activity of = 1 x 10^5 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 25 mM Tris, pH 7.6, 1.5 M sodium chloride
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 10mM Tris, pH 7.6, to 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
Centrifuge the vial prior to opening. Reconstitute in 10mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee