Target
FGF2 / Basic FGF
Synonyms
FGF2 | Basic fibroblast growth factor | FGFB | Fibroblast growth factor 2 | FGF-2 | Prostatropin | BFGF | HBGF-2
Modifications
Animal-free.
Also available
Unmodified.
Conjugations
Unconjugated
AA Sequence
AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Expression System
E. coli
Purification
Greater than 95% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by a cell proliferation Assay using balb/c 3T3 cells is = 0.1 ng/ml, corresponding to a specific activity of = 1 x 10^7 units/mg.
Endotoxin
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Presentation
Sterile filtered through a 0.2-micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.4 + 112.5mM NaCl
Reconstitution
Centrifuge vial prior to opening. Do not vortex after reconstitution. Avoid repeated freeze-thaw cycles. Initially reconstitute in water to 0.1-1.0 mg/ml.
Storage
After reconstitution store at 2°C to 8°C for up to 1 week or for extended storage further dilute in a buffer containing a carrier protein or stabilizer (e.g. 5% Trehalose). Store working aliquots at -20°C to -80°C for a year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee