Target
FGF1 / Acidic FGF
Synonyms
FGF1 | AFGF | Endothelial cell growth factor | Fibroblast growth factor 1 | FGFA | ECGFB | HBGF-1 | GLIO703 | ECGF | ECGF-beta | ECGFA | FGF-1 | FGF-alpha | HBGF1
Modifications
Unmodified.
Also available
Animal-free.
Conjugations
Unconjugated
Predicted Molecular Weight
16.0 kDa
AA Sequence
MFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Expression System
E. coli
Purification
Greater than 95% by SDS-PAGE and HPLC
Bio-Activity
The ED50 as determined by a cell proliferation assay using Balb/c 3T3 cells is 0.5 ng/ml in the presence of 10 µg/ml heparin, corresponding to a specific activity of = 2 x 10^6 units/mg.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 5 mM sodium phosphate, pH 8.0, 100 mM sodium chloride
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate pH 8.0 to a concentration of 0.5 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee