Target
CD27
Synonyms
CD27 | CD27 antigen | CD27 molecule | S152 | T14 | T-cell activation antigen CD27 | CD27L receptor | T cell activation antigen S152 | TNFRSF7 | Tp55
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Rabbit IgG Fc, C-terminus
Predicted Molecular Weight
The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized human CD27-his at 10 µg/mL (100 µl/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee